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advances in small molecule fluorescent probes for simultaneous imaging of two bioactive molecules in live cellsand in vivo

Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 4-33 doi: 10.1007/s11705-021-2041-2

摘要： The interrelationships and synergistic regulations of bioactive molecules play pivotal roles in physiological and pathological processes involved in the initiation and development of some diseases, such as cancer and neurodegenerative and cardiovascular diseases. Therefore, the simultaneous, accurate and timely detection of two bioactive molecules is crucial to explore their roles and pathological mechanisms in related diseases. Fluorescence imaging associated with small molecular probes has been widely used in the imaging of bioactive molecules in living cells and due to its excellent performances, including high sensitivity and selectivity, noninvasive properties, real-time and high spatial temporal resolution. Single organic molecule fluorescent probes have been successively developed to simultaneously monitor two biomolecules to uncover their synergistic relationships in living systems. Hence, in this review, we focus on summarizing the design strategies, classifications, and bioimaging applications of dual-response fluorescent probes over the past decade. Furthermore, future research directions in this field are proposed.

关键词： bioactive molecules     fluorescent probes     in living cells and in vivo     review    

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A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in livingcells and tissues

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 103-111 doi: 10.1007/s11705-021-2064-8

摘要： Uridine diphosphate (UDP)-glucuronosyltransferases (UGTs) are enzymes involved in the biotransformation of important endogenous compounds such as steroids, bile acids, and hormones as well as exogenous substances including drugs, environmental toxicants, and carcinogens. Here, a novel fluorescent probe BDMP was developed based on boron-dipyrromethene (BODIPY) with high sensitivity for the detection of UGT1A8. The glucuronidation of BDMP not only exhibited a red-emission wavelength (λex/λem = 500/580 nm), but also displayed an excellent UGT1A8-dependent fluorescence signal with a good linear relationship with UGT1A8 concentration. Based on this perfect biocompatibility and cell permeability, BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines (LoVo and HCT15) in real time. In addition, BDMP could also be used to visualize UGT1A8 in tumor tissues. These results suggested that BDMP is a promising molecular tool for the investigation of UGT1A8-mediated physiological function in humans.

关键词： UDP-glucuronosyltransferase 1A8     fluorescent probe     subtype selectivity     fluorescence imaging    

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In vivo imaging of hematopoietic stem cell development in the zebrafish

Panpan Zhang, Feng Liu

《医学前沿（英文）》 2011年 第5卷 第3期   页码 239-247 doi: 10.1007/s11684-011-0123-0

摘要： imaging is crucial for developmental biology and can further help to follow cell development/differentiation in normal and pathological conditions. Recent advances in optical imaging techniques has facilitated tracing of the developmental dynamics of a specific organ, tissue, or even a single cell. The zebrafish is an excellent model for imaging of hematopoiesis due to its transparent embryo at early stage; moreover, different zebrafish hematopoietic stem cells (HSCs) transgenic lines have been demonstrated as very useful tools for illustrating the details of the HSC developmental process. In this review, we summarize recent studies related to the non-invasive imaging of HSC transgenics, to show that zebrafish transgenic lines are powerful tools for developmental biology and disease. At the end of the review, the perspective and some open questions in this field will be discussed.

关键词： hematopoietic stem cell     hematopoiesis     in vivo imaging     transgenics     zebrafish    

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The comparative study of dwelling houses in the southwest multi-ethnic region, taking Fugong county for example

Fang WANG, Jiaping LIU

《结构与土木工程前沿（英文）》 2010年 第4卷 第4期   页码 474-482 doi: 10.1007/s11709-010-0089-z

摘要： A number of ethnic minorities are being distributed in southwest China. Yunnan Province is an area inhabited by multi-ethnic groups. For a long time, the ethnic groups have been interacted by others in the cultural aspect, while retaining their own ethnic traditions. The performances of ethnic groups’ dwelling houses are similar as determined by natural and cultural elements of the same area. This paper mainly analyzes decisive factors behind similar appearances by the way of field research on two minorities, Nu ethnic group and Li-su ethnic group, in the Fugong county, Nujiang Li-su autonomous prefecture. Moreover, the paper tries to summarize the commonality and individuality of the minorities in order to provide useful theoretical reference for the minorities building of new socialist countryside in the future.

关键词： Nu ethnic group     Li-su ethnic group     mixed region     living culture     living mode     living acts    

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Working Up Green, and Living Up Green

Ji-shan He

《工程管理前沿（英文）》 2014年 第1卷 第1期   页码 2-2 doi: 10.15302/J-FEM-2014002

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Characterising populations living close to intensive farming and composting facilities in England

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1332-z

摘要：

• Bioaerosol emitted from farming and composting facilities may pose health risks.

关键词： Composting     Intensive farming     Bioaerosol     Population characteristics     Public health    

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21世纪人类生活与生存的科技

蒋正华

《中国工程科学》 2002年 第4卷 第4期   页码 1-11

摘要：

从管理学角度回顾了过去百年的世界历史，研究新世纪可能发生的进步，并探讨更好地建立 世界和平与合作，实现共同发展的方案。

关键词： 21世纪     科技进步     人类生活与生存    

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SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Article

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

《工程（英文）》 doi: 10.1016/j.eng.2023.05.010

摘要：

Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.

关键词： In vivo     Short-wave infrared     Skeletal muscle     Macrophage     Regeneration    

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开发浅海滩涂资源 拓展我国的生存空间

陈吉余

《中国工程科学》 2000年 第2卷 第3期   页码 27-31

摘要：

据预测21世纪中期我国人口将净增2〜3亿，而耕地减少累计量将达1800万hm2,二者叠加到21世纪中叶我国将缺少4〜5亿人口的生存空间，人口、资源、环境形势非常严峻；而如何拓展生存空间的形势也是极其严重的，河流上游封山育林，中游退田还湖，但沿海滩涂却可提供大量后备土地资源。历史上我国退海、围海之地达10余万km2。近50年来，加大围海造地力度，新增了11000〜12000km2土地。本文提出2050年前再造10000〜15000km2生存空间，为2000〜3000万人提供就业机会，还着重提出未来50年围垦难点所在是低滩围垦。根据人海泥沙自然造陆与所需造陆之间的差值，必需以人工促淤和生物促淤工程来弥补自然成陆泥沙之不足。还需在提高环境监测、预测的精度，以及工程技术等方面的技术创新。

关键词： 浅海滩涂资源     生存空间     围海     人工海岸     促淤工程    

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蓝色海洋生物资源开发战略研究

“中国海洋工程与科技发展战略研究”海洋生物资源课题组

《中国工程科学》 2016年 第18卷 第2期   页码 32-40 doi: 10.15302/J-SSCAE-2016.02.004a

摘要：

为了提升海洋生物资源的开发能力，推进海洋强国的建设，中国工程院于2011年启动了“中国海洋生物资源工程与科技发展战略研究”重大咨询课题研究。课题从海水养殖、资源养护、远洋渔业、质量安全与加工流通、海洋药物与生物制品五个重要领域开展咨询调查研究，系统分析研究了发展现状、趋势和战略需求，提出了多层面地开发利用海洋生物资源的“养护、拓展、高技术”三大发展战略和推进蓝色海洋生物产业“可持续、安全、现代工程化”发展的三大战略目标及其任务，建议国家优先启动“蓝色海洋食物开发工程”和“蓝色海洋药物与生物制品关键技术开发工程”两个重点研发专项。

关键词： 蓝色海洋     生物资源开发     发展战略     研发专项    

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Fabrication and in vivo evaluation of Ti6Al4V implants with controlled porous structure and complex shape

Xiang LI, Yun LUO, Chengtao WANG, Wenguang ZHANG, Yuanchao LI

《机械工程前沿（英文）》 2012年 第7卷 第1期   页码 66-71 doi: 10.1007/s11465-012-0302-y

摘要：

Electron beam melting process was used to fabricate porous Ti6Al4V implants. The porous structure and surface topography of the implants were characterized by scanning electron microscopy (SEM) and digital microscopy (DM). The results showed that the pore size was around 600 and the porosity approximated to 57%. There was about±50 μm of undulation on implants surfaces. Standard implants and a custom implant coupled with porous sections were designed and fabricated to validate the versatility of the electron beam melting (EBM) technique. After coated with bone-like apatite, samples with fully porous structures were implanted into cranial defects in rabbits to investigate the in vivo performance. The animals were sacrificed at 8 and 12 weeks after implantation. Bone ingrowth into porous structure was examined by histological analysis. The histological sections indicated that a large amount of new bone formation was observed in porous structure. The newly formed bone grew from the calvarial margins toward the center of the bone defect and was in close contact with implant surfaces. The results of the study showed that the EBM produced Ti6Al4V implants with well-controlled porous structure, rough surface topography and bone-like apatite layer are beneficial for bone ingrowth and apposition.

关键词： electron beam melting process     implant     porous structure     bone ingrowth    

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复杂信息系统构建的新方法——多活性代理方法

王越

《中国工程科学》 2006年 第8卷 第5期   页码 29-32

摘要： 在分析复杂信息系统的基础上，以系统理论为指导，借用多代理技术的思想，针对强对抗、强约束条件下复杂信息系统的特点，提出了复杂信息系统构建的一种新方法——多活性代理方法（MLAM, multi living

关键词： 多活性代理方法     复杂信息系统     系统理论    

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Reducing biliary complications in adult-to-adult living donor liver transplantation using right lobe

YAN Lunan, WEN Tianfu, WANG Wentao, YANG Jiayin, XU Mingqing, CHEN Zheyu, WU Hong

《医学前沿（英文）》 2008年 第2卷 第2期   页码 130-133 doi: 10.1007/s11684-008-0024-z

摘要： The aim of this paper is to summarize our experience of using right lobe liver grafts to reduce biliary complications in adult-to-adult (A-A) living donor liver transplantation (LDLT). From January 2002 to October 2007, 124 adult patients underwent living donor liver transplantation using right lobe grafts at the West China Hospital, Sichuan University Medical School, China. There was no death in all donors. Biliary reconstruction for 178 hepatic duct orifices from 124 donor grafts was performed which included 106 reconstructions of duct-to-duct anastomoses and 72 cholangiojejunostomy. Nine recipients had biliary complications including six bile leakages (four from the anastomotic site and two from the cut surface of the liver graft) and three biliary strictures. With the improved techniques for biliary reconstruction, we have achieved good results in 124 recipients of A-A LDLT. We ascribe our success to the introduction of microsurgical techniques and the use of fixed operators which help in decreasing the biliary complications of LDLT.

关键词： microsurgical     October     improved     success     transplantation    

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Preliminary experience in adult-to-adult living donor liver transplantation in a single center in China

YAN Lunan, ZENG Yong, WEN Tianfu, ZHAO Jichun, WANG Wentao, YANG Jiayin, XU Mingqing, MA Yukui, CHEN Zheyu, LIU Jiangwen, WU Hong

《医学前沿（英文）》 2007年 第1卷 第2期   页码 136-141 doi: 10.1007/s11684-007-0026-2

摘要： The aim of this paper is to report the authors experience in performing adult-to-adult living donor liver transplantation (LDLT) by using a modified technique in using grafts of the right lobe of the liver. From January 2002 to September 2006, 56 adult patients underwent LDLT using right lobe grafts at the West China Hospital, Sichuan University Medical School, China. All patients underwent a modified operation designed to improve the reconstruction of the right hepatic vein (RHV) and the tributariers of the middle hepatic vein (MHV) by interposing a vessel graft, and by anastomosing the hepatic arteries and bile ducts. There were no severe complications or deaths in all donors. Fifty-two (92.8%) recipients survived the operations. Among the 56 recipients, complications were seen in 15 recipients (26.8%), including hepatic vein stricture (one case), small-for-size syndrome (one case), hepatic artery thrombosis (two cases), intestinal bleeding (one case), bile leakage (two cases), left subphrenic abscess (one case), renal failure (two cases) and pulmonary infection (five cases). Within three months after transplantation, four recipients (7.14%) died due to smallfor-size syndrome (one case), renal failure (one case) and multiple organ failure (two cases). All patients underwent direct anastomoses of the RHV and the inferior vena cava (IVC), and in 23 cases, reconstruction of the right inferior hepatic vein was also done. In 24 patients, the reconstruction of the tributaries of the MHV was also done by interposing a vessel graft to provide sufficient venous outflow. Trifurcation of the portal vein was seen in nine cases. Thus, veno-plasty or separate anastomoses were performed. The graft and recipient body weight ratios (GRWR) were between 0.72% and 1.43%, and in three cases it was <0.8%. The graft weight to recipient standard liver volume ratios (GV/SLV) were between 31.86% and 71.68%, among which four cases had <40%. No small-for-size syndrome occurred. With modification of the surgical technique, especially in the reconstruction of the hepatic vein to provide sufficient venous outflow, living donor liver grafts in adults using the right lobe of the liver can become a relatively safe procedure and prevent the small-for-size syndrome .
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Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probes for in vivo

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 64-71 doi: 10.1007/s11705-021-2048-8

摘要： Heterocyclic compound quinoline and its derivatives exist in natural compounds and have a broad spectrum of biological activity. They play an important role in the design of new structural entities for medical applications. Similarly, indoles and their derivatives are found widely in nature. Amino acids, alkaloids and auxin are all derivatives of indoles, as are dyes, and their condensation with aldehydes makes it easy to construct reaction sites for nucleophilic addition agents. In this work, we combine these two groups organically to construct a rapid response site (within 30 s) for H S, and at the same time, a ratiometric fluorescence response is presented throughout the process of H S detection. As such, the lower detection limit can reach 55.7 nmol/L for H S. In addition, cell imaging shows that this probe can be used for the mitochondrial targeted detection of endogenous and exogenous H S. Finally, this probe application was verified by imaging H S in nude mice.

关键词： heterocyclic compound     hydrogen sulfide     ratiometric     mitochondrial targeted    

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标题 作者 时间 类型 操作

advances in small molecule fluorescent probes for simultaneous imaging of two bioactive molecules in live cellsand in vivo

Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li

期刊论文

A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in livingcells and tissues

期刊论文

In vivo imaging of hematopoietic stem cell development in the zebrafish

Panpan Zhang, Feng Liu

期刊论文

The comparative study of dwelling houses in the southwest multi-ethnic region, taking Fugong county for example

Fang WANG, Jiaping LIU

期刊论文

Working Up Green, and Living Up Green

Ji-shan He

期刊论文

Characterising populations living close to intensive farming and composting facilities in England

期刊论文

21世纪人类生活与生存的科技

蒋正华

期刊论文

SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

期刊论文

开发浅海滩涂资源 拓展我国的生存空间

陈吉余

期刊论文

蓝色海洋生物资源开发战略研究

“中国海洋工程与科技发展战略研究”海洋生物资源课题组

期刊论文

Fabrication and in vivo evaluation of Ti6Al4V implants with controlled porous structure and complex shape

Xiang LI, Yun LUO, Chengtao WANG, Wenguang ZHANG, Yuanchao LI

期刊论文

复杂信息系统构建的新方法——多活性代理方法

王越

期刊论文

Reducing biliary complications in adult-to-adult living donor liver transplantation using right lobe

YAN Lunan, WEN Tianfu, WANG Wentao, YANG Jiayin, XU Mingqing, CHEN Zheyu, WU Hong

期刊论文

Preliminary experience in adult-to-adult living donor liver transplantation in a single center in China

YAN Lunan, ZENG Yong, WEN Tianfu, ZHAO Jichun, WANG Wentao, YANG Jiayin, XU Mingqing, MA Yukui, CHEN Zheyu, LIU Jiangwen, WU Hong

期刊论文

Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probes for in vivo

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

期刊论文